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Overview
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Collection
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NIA Aging Cell Culture Repository
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| Subcollection |
Specially Characterized Fibroblasts |
| Sample Description |
IMR-90 - SV40 VIRUS-TRANSFORMED FETAL LUNG FIBROBLAST |
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Biopsy Source
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Lung
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Cell Type
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Fibroblast
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Tissue Type
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Lung
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Transformant
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Simian Virus 40
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Species
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Homo sapiens
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Common Name
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Human
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Age At Sampling
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16 FW
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Sex
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Female
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Race
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Caucasian
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Relation to Proband
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proband
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Clinically Affected
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No
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Confirmation
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Clinical summary/Case history
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ISCN
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41,X>69,XXX, multiple rearrangements cp[10]
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Remarks
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| Catalog ID |
AG02804 |
| Product |
Cell Culture |
| Pricing |
Commercial Pricing: $180.00
Academic and not-for-profit pricing: $100.00
NIA Grantees: $40.00 |
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| Catalog ID |
NG02804 |
| Product |
DNA |
| Quantity |
10ug |
| Source |
cell culture |
| Pricing |
Commercial Pricing: $140.00
Academic and not-for-profit pricing: $60.00
NIA Grantees: $50.00 |
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How to Order
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Online Ordering
Assurance Form (Must have current form on file)
Statement of Research Intent Form (Information will be entered electronically when order is placed. DO NOT fax form to Coriell Customer Service)
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Characterizations
| Sample Description |
IMR-90 - SV40 VIRUS-TRANSFORMED FETAL LUNG FIBROBLAST |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis DNA fingerprinting and HLA DQ-alpha typing showed that this post-crisis SV40 transformed fibroblast culture had the same pattern and genotype as IMR90, the untransformed fibroblast. |
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| HLA TYPING |
Analysis of HLA DQ-alpha using the AmpliType forensic DNA amplification and typing kit showed the HLA DQ-alpha genotype for this virustransformed human cell line to be 3,3. |
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Phenotypic Data
| Remark |
AG02804D is a post-crisis SV40-transformed IMR-90 culture. It was derived from the same pool of infected cells that initiated the pre-crisis transformed IMR-90 culture, AG03204A. Both cultures were initiated by infecting IMR-90 at PDL 30 with SV40 virus strain Rh 911. AG02804D contains 100% T antigen positive cells and 1% to 5% V-antigen positive cells. The cell morphology is epithelial-like. The karyotype is 41,X>69,XXXX, multiple rearrangements cp[10]. |
Publications
| Takemura H, Rao VA, Sordet O, Furuta T, Miao ZH, Meng L, Zhang H, Pommier Y, Defective Mre11-dependent activation of Chk2 by ataxia telangiectasia mutated in colorectal carcinoma cells in response to replication-dependent DNA double strand breaks The Journal of biological chemistry281:30814-23 2006 |
| PubMed ID: 16905549 |
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| Gosslau A, Chen M, Ho CT, Chen KY, A methoxy derivative of resveratrol analogue selectively induced activation of the mitochondrial apoptotic pathway in transformed fibroblasts Br J Cancer92(3):513-21 2005 |
| PubMed ID: 15668717 |
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| Makarov VL, Hirose Y, Langmore JP, Long G tails at both ends of human chromosomes suggest a C strand degradation mechanism for telomere shortening. Cell88:657-66 1997 |
| PubMed ID: 9054505 |
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| Duan C, Clemmons DR, Transcription factor AP-2 regulates human insulin-like growth factor binding protein-5 gene expression. J Biol Chem270:24844-51 1995 |
| PubMed ID: 7559606 |
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| Schaefer DI, Livanos EM, White AE, Tlsty TD, Multiple mechanisms of N-(phosphonoacetyl)-L-aspartate drug resistance in SV40-infected precrisis human fibroblasts. Cancer Res53:4946-51 1993 |
| PubMed ID: 8402685 |
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| Camacho-Hubner C, Busby WH Jr, McCusker RH, Wright G, Clemmons DR, Identification of the forms of insulin-like growth factor-binding proteins produced by human fibroblasts and the mechanisms that regulate their secretion. J Biol Chem267:11949-56 1992 |
| PubMed ID: 1376315 |
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| Saito H, Moses RE, Immortalization of Werner syndrome and progeria fibroblasts Experimental cell research192:373-9 1991 |
| PubMed ID: 1671011 |
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| Magnuson VL, Young M, Schattenberg DG, Mancini MA, Chen DL, Steffensen B, Klebe RJ, The alternative splicing of fibronectin pre-mRNA is altered during aging and in response to growth factors. J Biol Chem266:14654-62 1991 |
| PubMed ID: 1713586 |
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| Wood CM, Moses RE, Ethyl methane sulfonate- and bleomycin-generated deletion mutations at HPRT locus in xeroderma pigmentosum complementation group D fibroblasts. Somat Cell Mol Genet15:345-57 1989 |
| PubMed ID: 2474861 |
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| Saitoh T, Sundsmo M, Roch JM, Kimura N, Cole G, Schubert D, Oltersdorf T, Schenk DB, Secreted form of amyloid beta protein precursor is involved in the growth regulation of fibroblasts. Cell58:615-22 1989 |
| PubMed ID: 2475254 |
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| Baldwin GC, Gasson JC, Kaufman SE, Quan SG, Williams RE, Avalos BR, Gazdar AF, Golde DW, DiPersio JF, Nonhematopoietic tumor cells express functional GM-CSF receptors. Blood73:1033-7 1989 |
| PubMed ID: 2537665 |
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| Gibson JM, Milam SB, Klebe RJ, Late passage cells display an increase in contractile behavior. Mech Ageing Dev48:101-10 1989 |
| PubMed ID: 2739467 |
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| McNearney T, Ballard L, Seya T, Atkinson JP, Membrane cofactor protein of complement is present on human fibroblast, epithelial, and endothelial cells. J Clin Invest84:538-45 1989 |
| PubMed ID: 2474570 |
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| Hara H, Kaji H, Random integration of SV40 in SV40-transformed, immortalized human fibroblasts. Exp Cell Res168:531-8 1987 |
| PubMed ID: 3026824 |
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| Wood CM, Timme TL, Hurt MM, Brinkley BR, Ledbetter DH, Moses RE, Transformation of DNA repair-deficient human diploid fibroblasts with a simian virus 40 plasmid. Exp Cell Res169:543-53 1987 |
| PubMed ID: 3030788 |
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| Wilhelm SM, Collier IE, Kronberger A, Eisen AZ, Marmer BL, Grant GA, Bauer EA, Goldberg GI, Human skin fibroblast stromelysin: structure, glycosylation, substrate specificity, and differential expression in normal and tumorigenic cells. Proc Natl Acad Sci U S A84:6725-9 1987 |
| PubMed ID: 3477804 |
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| Stein GH, Atkins L, Beeson M, Gordon L, Quiescent human diploid fibroblasts. Common mechanism for inhibition of DNA replication in density-inhibited and serum-deprived cells. Exp Cell Res162:255-60 1986 |
| PubMed ID: 2998845 |
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| Bunn CL, Baron JI, Mitchell D, Inherent resistance of HeLa cell derivatives to paromomycin. In Vitro Cell Dev Biol22:381-6 1986 |
| PubMed ID: 3015861 |
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| Ballard FJ, Regulation of protein breakdown by epidermal growth factor in A431 cells. Exp Cell Res157:172-80 1985 |
| PubMed ID: 3882435 |
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| Fukuchi K, Tanaka K, Nakura J, Kumahara Y, Uchida T, Okada Y, Elevated spontaneous mutation rate in SV40-transformed Werner syndrome fibroblast cell lines. Somat Cell Mol Genet11:303-8 1985 |
| PubMed ID: 2992100 |
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| Stein GH, SV40-transformed human fibroblasts: evidence for cellular aging in pre- crisis cells. J Cell Physiol125:36-44 1985 |
| PubMed ID: 2995426 |
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| Ballard FJ, The epidermal growth factor-mediated inhibition of growth in A431 cells is accompanied by an atypical stimulation of protein breakdown. Exp Cell Res151:268-72 1984 |
| PubMed ID: 6321213 |
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| Chung HR, Albanese EA, Studzinski GP, Differential effect of coformycin on the cell cycle traverse of normal and simian virus 40-transformed human fibroblasts. Cancer Res43:1269-74 1983 |
| PubMed ID: 6297723 |
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| Fox N, Studzinski GP, DNA dependence and inhibition by novobiocin and coumermycin of the nucleolar adenosine triphosphatase (ATPase) of human fibroblasts. J Histochem Cytochem30:364-70 1982 |
| PubMed ID: 6460802 |
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| Studzinski, DNA-dependent nATPase associated with intranucleolar chromatin of human cells in culture. J Cell Biol91:67a (1981):364-70 1981 |
| PubMed ID: 6460802 |
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| Fox N, Fernandez C, Studzinski GP, Visualization of nucleolar substructure in cultured human fibroblasts by magnesium-activated adenosine triphosphatase reaction. J Histochem Cytochem29:1115-20 1981 |
| PubMed ID: 6117591 |
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| Stein GH, Yanishevsky RM, Quiescent human diploid cells can inhibit entry into S phase in replicative nuclei in heterodikaryons. Proc Natl Acad Sci U S A78:3025-9 1981 |
| PubMed ID: 6265932 |
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| Tunkel AR, Studzinski GP, Effect of aminonucleoside on transcription, methylation, and maturation of ribosomal RNA in SV40-transformed human lung fibroblasts. J Cell Physiol108:239-49 1981 |
| PubMed ID: 6267084 |
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| Studzinski GP, Albanese EA, Nascent RNA chain termination and ultrastructural changes in nucleoli isolated from SV40-transformed fibroblasts treated with aminonucleoside of puromycin. Lab Invest43:427-33 1980 |
| PubMed ID: 6158625 |
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| Ballard FJ, Wong SS, Knowles SE, Partridge NC, Martin TJ, Wood CM, Gunn JM, Insulin inhibition of protein degradation in cell monolayers. J Cell Physiol105:335-46 1980 |
| PubMed ID: 7007398 |
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| Studzinski GP, Albanese EA, Scatina J, Trocola-Simchera M, Fibroblasts transformed by an oncogenic virus show decreased uptake of puromycin aminonucleoside. Biochem Biophys Res Commun95:1446-51 1980 |
| PubMed ID: 6251841 |
Culture Protocols
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
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