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Overview
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Collection
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NIA Aging Cell Culture Repository
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| Subcollection |
Specially Characterized Fibroblasts |
| Sample Description |
MRC-5 - NORMAL HUMAN FETAL LUNG FIBROBLAST |
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Biopsy Source
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Lung
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Cell Type
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Fibroblast
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Tissue Type
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Lung
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Transformant
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Untransformed
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Species
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Homo sapiens
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Common Name
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Human
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Age
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14 FW
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Sex
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Male
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Race
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Caucasian
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Relation to Proband
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proband
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Clinically Affected
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No
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY
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Remarks
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| Catalog ID |
AG05965-F |
| Product |
Cell Culture |
| Pricing |
Commercial Pricing: $180.00
Academic and not-for-profit pricing: $100.00
NIA Grantees: $40.00 |
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How to Order
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Online Ordering
Assurance Form (Must have current form on file)
Statement of Research Intent Form (Information will be entered electronically when order is placed. DO NOT fax form to Coriell Customer Service)
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Characterizations
| Sample Description |
MRC-5 - NORMAL HUMAN FETAL LUNG FIBROBLAST |
| PDL at Freeze |
16.99 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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Phenotypic Data
| Remark |
The MRC-5 cell line was developed in September 1966 from lung tissue taken from a 14 week fetus aborted for psychiatric reason from a 27 year old physically healthy woman. The cell morphology is fibroblast-like. The karyotype is 46,XY; normal diploid male. Cumulative population doublings to senescence is 42-48. G6PD isoenzyme is type B. |
Publications
| Guo WJ, Datta S, Band V, Dimri GP, Mel-18, a polycomb group protein, regulates cell proliferation and senescence via transcriptional repression of Bmi-1 and c-Myc oncoproteins Molecular biology of the cell18:536-46 2006 |
| PubMed ID: 17151361 |
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| Von Kobbe C, May A, Grandori C, Bohr VA, Werner syndrome cells escape hydrogen peroxide-induced cell proliferation arrest. FASEB J18(15):1970-2 2004 |
| PubMed ID: 15459124 |
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| Skandalis A, Uribe E, A survey of splice variants of the human hypoxanthine phosphoribosyl transferase and DNA polymerase beta genes: products of alternative or aberrant splicing? Nucleic Acids Res32(22):6557-64 2004 |
| PubMed ID: 15601998 |
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| Hande MP, Balajee AS, Tchirkov A, Wynshaw-Boris A, Lansdorp PM, Extra-chromosomal telomeric DNA in cells from Atm(-/-) mice and patients with ataxia-telangiectasia. Hum Mol Genet10(5):519-28 2001 |
| PubMed ID: 11181576 |
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| Gyulai Z, Endresz V, Burian K, Pincus S, Toldy J, Cox WI, Meric C, Plotkin S, Gonczol E, Berencsi K, Cytotoxic T lymphocyte (CTL) responses to human cytomegalovirus pp65, IE1-Exon4, gB, pp150, and pp28 in healthy individuals: reevaluation of prevalence of IE1-specific CTLs. J Infect Dis181(5):1537-46 2000 |
| PubMed ID: 10823751 |
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| Piret B, Schoonbroodt S, Piette J, The ATM protein is required for sustained activation of NF-kappaB following DNA damage. Oncogene18(13):2261-71 1999 |
| PubMed ID: 10327072 |
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| Jongmans W, Vuillaume M, Chrzanowska K, Smeets D, Sperling K, Hall J, Nijmegen breakage syndrome cells fail to induce the p53-mediated DNA damage response following exposure to ionizing radiation. Mol Cell Biol17(9):5016-22 1997 |
| PubMed ID: 9271379 |
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| Duan C, Clemmons DR, Transcription factor AP-2 regulates human insulin-like growth factor binding protein-5 gene expression. J Biol Chem270:24844-51 1995 |
| PubMed ID: 7559606 |
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| Borsi L, Balza E, Gaggero B, Allemanni G, Zardi L, The alternative splicing pattern of the tenascin-C pre-mRNA is controlled by the extracellular pH. J Biol Chem270:6243-5 1995 |
| PubMed ID: 7534307 |
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| Futreal PA, Cochran C, Marks JR, Iglehart JD, Zimmerman W, Barrett JC, Wiseman RW, Mutation analysis of the THRA1 gene in breast cancer: deletion/fusion of the gene to a novel sequence on 17q in the BT474 cell line. Cancer Res54:1791-4 1994 |
| PubMed ID: 7511052 |
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| Kilfeather SA, Collins D, McCormack P, Cotter T, O'Malley K, The effect of in vitro aging on human lung fibroblast beta-adrenergic receptor density, coupling and response. Mech Ageing Dev63:247-56 1992 |
| PubMed ID: 1319530 |
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| Jacobs, Characteristics of a human diploid cell designated MRC-5. Nature277:168 (1970):247-56 1970 |
| PubMed ID: 1319530 |
Protocols
| PDL at Freeze |
16.99 |
| Split Ratio |
1:7 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
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