AG19284
Fibroblast from Tendon, Leg
Description:
NON WOUND HEALING PHENOTYPE
Repository
|
NIA Aging Cell Culture Repository
|
Subcollection |
Non-Healing Wound Collection |
Biopsy Source
|
Leg
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Tendon
|
Transformant
|
Untransformed
|
Sample Source
|
Fibroblast from Tendon, Leg
|
Race
|
White
|
Family Member
|
1
|
Relation to Proband
|
proband
|
Confirmation
|
Clinical summary/Case history
|
ISCN
|
46,XY
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
PDL at Senescence |
42 |
Passage Frozen |
3 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
|
Remarks |
Culture established from cells from non-healing wound of the left calf; a history of non-healing venous ulcer with infected with Pseudomonas and Staphlococcus; donor subject has on his left calf an upper smaller anterior wound of 3 months duration and a lower larger wound whose duration is years; multiple compound fracture to leg 14 years earlier; multiple failed skin grafts; mildly obese; occasional marijuana use; no diabetes; hypertensive; culture derived from tendon from the lower wound; history of non-healing venous ulcer; subject on intravenous ancef and oral ciprofloxacin; AG19282, AG19285 and AG 19286 were prepared from the same donor. |
Brem H, Golinko MS, Stojadinovic O, Kodra A, Diegelmann RF, Vukelic S, Entero H, Coppock DL, Tomic-Canic M, Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and test putative growth factor therapy such as GMCSF Journal of translational medicine6:75 2008 |
PubMed ID: 19046453 |
PDL at Senescence |
42 |
Passage Frozen |
3 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
- |
|
|