AG19289
Fibroblast from Skin, Leg
Description:
NON WOUND HEALING PHENOTYPE
Repository
|
NIA Aging Cell Culture Repository
|
Subcollection |
Non-Healing Wound Collection |
Biopsy Source
|
Leg
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Skin
|
Transformant
|
Untransformed
|
Sample Source
|
Fibroblast from Skin, Leg
|
Race
|
White
|
Family Member
|
1
|
Relation to Proband
|
proband
|
Confirmation
|
Clinical summary/Case history
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
PDL at Freeze |
5 |
Passage Frozen |
3 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
|
Remarks |
Culture established from cells from non-healing calf wound complicated by infection; donor subject has on her left calf an upper anterior wound and a lower posterior wound; tissue was debrided from level two (deeper subcutaneous tissue) of the posterior wound; donor has type II diabetes; donor has calcified arteries in the limb with only mild peripheral arterial disease; venous reflux ulcers confirmed by ultrasound; on prednisone (10 mg/day) and intravenous ancef |
Brem H, Golinko MS, Stojadinovic O, Kodra A, Diegelmann RF, Vukelic S, Entero H, Coppock DL, Tomic-Canic M, Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and test putative growth factor therapy such as GMCSF Journal of translational medicine6:75 2008 |
PubMed ID: 19046453 |
Cumulative PDL at Freeze |
5 |
Passage Frozen |
3 |
Split Ratio |
1:3 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
Serum |
10% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
- |
|
|