AG22139
Fibroblast from Skin, Buttock
Description:
NON WOUND HEALING PHENOTYPE
Repository
|
NIA Aging Cell Culture Repository
|
Subcollection |
Non-Healing Wound Collection |
Biopsy Source
|
Buttock
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Skin
|
Transformant
|
Untransformed
|
Sample Source
|
Fibroblast from Skin, Buttock
|
Race
|
White
|
Family Member
|
1
|
Family History
|
N
|
Relation to Proband
|
proband
|
Confirmation
|
Clinical summary/Case history
|
ISCN
|
46,XY[26]/47,XY,+10[24]
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
PDL at Senescence |
27 |
PDL at Freeze |
3.74 |
Passage Frozen |
2 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
|
Remarks |
This culture was initiated from explants of minced tissue taken from the healing edge of a pressure ulcer on the left buttocks; stage 4 sacral ulcer; dementia; seizures; chronic anemia; dysphagia; relies on g-tube for eating |
Brem H, Golinko MS, Stojadinovic O, Kodra A, Diegelmann RF, Vukelic S, Entero H, Coppock DL, Tomic-Canic M, Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and test putative growth factor therapy such as GMCSF Journal of translational medicine6:75 2008 |
PubMed ID: 19046453 |
PDL at Senescence |
27 |
Cumulative PDL at Freeze |
3.74 |
Passage Frozen |
2 |
Split Ratio |
1:3 |
Temperature |
37 C |
Percent CO2 |
5% |
Percent O2 |
AMBIENT |
Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
Serum |
10% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
dilution - add fresh medium |
Supplement |
- |
|
|