AG22177
Fibroblast from Skin, Leg
Description:
NON WOUND HEALING PHENOTYPE
Repository
|
NIA Aging Cell Culture Repository
|
Subcollection |
Non-Healing Wound Collection |
Biopsy Source
|
Leg
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Skin
|
Transformant
|
Untransformed
|
Sample Source
|
Fibroblast from Skin, Leg
|
Race
|
Black/African American
|
Ethnicity
|
AFRICAN-AMERICAN
|
Family History
|
N
|
Relation to Proband
|
proband
|
Confirmation
|
Clinical summary/Case history
|
ISCN
|
47,XXX[5]/46,XX[45]
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
PDL at Senescence |
27 |
PDL at Freeze |
5.65 |
Passage Frozen |
2 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
|
Remarks |
Clinically affected; ex-smoker; valvular disease; hypertriglyceridemia; anxiety; hypertension; hysterectomy; left lower pretibial region with ulcer (15 x 15cm with fibrinous exudate); ulcer is shallow with no pus or erythema; exposed tendon and tenderness; culture established from explants of skin tissue removed at the time of wound debridement and skin grafting |
Brem H, Golinko MS, Stojadinovic O, Kodra A, Diegelmann RF, Vukelic S, Entero H, Coppock DL, Tomic-Canic M, Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and test putative growth factor therapy such as GMCSF Journal of translational medicine6:75 2008 |
PubMed ID: 19046453 |
PDL at Senescence |
27 |
Cumulative PDL at Freeze |
5.65 |
Passage Frozen |
2 |
Split Ratio |
1:5 |
Temperature |
37 C |
Percent CO2 |
5% |
Percent O2 |
AMBIENT |
Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
Serum |
10% fetal bovine serum Not inactivated |
Substrate |
None specified |
Supplement |
- |
|
|