GM10321
Somatic cell hybrid
Description:
ADDITIONAL HUMAN/MOUSE SOMATIC CELL HYBRIDS
Repository
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NIGMS Human Genetic Cell Repository
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Subcollection |
Hybrids |
Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Family Member
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17
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Confirmation
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Karyotypic analysis and Southern blot hybridization
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ISCN
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Human/Mouse Somatic Cell Hybrid retaining human chromosome #17
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Remarks
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IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for Xq28, DXS15. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 6 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 6pterp24, D6S7. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 12 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 12q14q24.1, D12S7. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 17p13.1, MYH2. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 21q22.1q22.2, D21S17. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 22q12.3q13.1, PDGFB. |
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Remarks |
Line TMS5; selected in HAT medium; produced by fusing HeLa S3 with LMTK- mouse cells; at P4 after recovery, 64% of cells have 1 human chromosome #17 and 11% have 2/cell; 21% of cells have 1 human #17p- chromosome and 4% have 2/cell |
Pironon N, Puechberty J, Roizès G, Molecular and evolutionary characteristics of the fraction of human alpha satellite DNA associated with CENP-A at the centromeres of chromosomes 1, 5, 19, and 21 BMC genomics11:195 2009 |
PubMed ID: 20331851 |
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Roizès G, Human centromeric alphoid domains are periodically homogenized so that they vary substantially between homologues Mechanism and implications for centromere functioning Nucleic acids research34:1912-24 2006 |
PubMed ID: 16598075 |
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Small KM, Brown KM, Seman CA, Theiss CT, Liggett SB, Complex haplotypes derived from noncoding polymorphisms of the intronless alpha2A-adrenergic gene diversify receptor expression Proceedings of the National Academy of Sciences of the United States of America103:5472-7 2006 |
PubMed ID: 16567612 |
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MacCollin M, Peterfreund R, MacDonald M, Fink JS, Gusella J, Mapping of a human A2a adenosine receptor (ADORA2) to chromosome 22. Genomics20:332-3 1994 |
PubMed ID: 8020991 |
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Howe JR, Lairmore TC, Veile R, Dou S, Wells SA Jr, Donis-Keller H, Development of a sequence-tagged site for the centromere of chromosome 10: its use in cytogenetic and physical mapping. Hum Genet91:199-204 1993 |
PubMed ID: 8478002 |
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Soussi-Yanicostas N, Whalen RG, Petit C, Five skeletal myosin heavy chain genes are organized as a multigene complex in the human genome. Hum Mol Genet2:563-9 1993 |
PubMed ID: 8518795 |
Split Ratio |
1:5 |
Temperature |
37 C |
Percent CO2 |
8% |
Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
Serum |
10% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
Aminopterin 4 x 10-7M |
Supplement |
Hypoxanthine 1 x 10-4M |
Supplement |
Thymidine 1.6 x 10-5M |
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