GM21309
LCL from B-Lymphocyte
Description:
INTERNATIONAL HAPMAP PROJECT - MAASAI IN KINYAWA, KENYA
INTERNATIONAL HAPMAP PROJECT - MAASAI IN KINYAWA, KENYA (PLATE I)
Repository
|
NHGRI Sample Repository for Human Genetic Research
|
Subcollection |
NHGRI Sample Repository for Human Genetic Research |
Biopsy Source
|
Peripheral vein
|
Cell Type
|
B-Lymphocyte
|
Tissue Type
|
Blood
|
Transformant
|
Epstein-Barr Virus
|
Sample Source
|
LCL from B-Lymphocyte
|
Country of Origin
|
KENYA
|
Family Member
|
1
|
Relation to Proband
|
child
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
|
Remarks |
At least three out of four grandparents were Maasai |
Vollger MR, Dishuck PC, Harvey WT, DeWitt WS, Guitart X, Goldberg ME, Rozanski AN, Lucas J, Asri M, Human Pangenome Reference Consortium M, Munson KM, Lewis AP, Hoekzema K, Logsdon GA, Porubsky D, Paten B, Harris K, Hsieh P, Eichler EE, Increased mutation and gene conversion within human segmental duplications Nature617:325-334 2022 |
PubMed ID: 37165237 |
Split Ratio |
1:3 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not Inactivated |
Substrate |
None specified |
Subcultivation Method |
dilution - add fresh medium |
Supplement |
- |
|
|