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Overview
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Collection
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NIA Aging Cell Culture Repository
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| Subcollection |
IMR90/IMR91 Cell Cultures
Specially Characterized Fibroblasts |
| Sample Description |
IMR-91 - NORMAL HUMAN FETAL FIBROBLAST |
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Biopsy Source
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Lung
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Cell Type
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Fibroblast
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Tissue Type
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Lung
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Transformant
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Untransformed
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Species
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Homo sapiens
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Common Name
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Human
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Age At Sampling
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12 FW
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Sex
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Male
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Race
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Caucasian
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Relation to Proband
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proband
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Clinically Affected
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No
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Confirmation
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Clinical summary/Case history
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Remarks
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| Catalog ID |
I91L-12 |
| Product |
Cell Culture |
| Pricing |
Commercial Pricing: $180.00
Academic and not-for-profit pricing: $100.00
NIA Grantees: $40.00 |
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| Catalog ID |
NAIMR91 |
| Product |
DNA |
| Quantity |
0.050mg |
| Source |
cell culture |
| Pricing |
Commercial Pricing: $55.00
Academic and not-for-profit pricing: $55.00
NIA Grantees: $55.00 |
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How to Order
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Online Ordering
Assurance Form (Must have current form on file)
Statement of Research Intent Form (Information will be entered electronically when order is placed. DO NOT fax form to Coriell Customer Service)
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Characterizations
| Sample Description |
IMR-91 - NORMAL HUMAN FETAL FIBROBLAST |
| PDL at Freeze |
19 |
| Passage Frozen |
7 |
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| GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME |
PCR analysis of DNA from this cell culture gave a positive result with a primer for Yq11, DYS227. |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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Phenotypic Data
| Remark |
The IMR-91 lung-derived line was initiated on 2/24/76 from explants of minced tissue of both lungs from an approximately 12 week fetus. The cell morphology is fibroblast-like. The karyotype is 46,XY; normal diploid male. The reported number of population doublings achieved to senescence range from 49 to 76. The HLA typing is A2, A11, B18 and B5. G6PD is isoenzyme type B and 6PGD is isoenzyme type A. |
Publications
| Sagesaka T, Boubnov N, Okuyama T, Paulus H, Sarkar N, Deoxyribonucleic acid replication in fetal cells. Am J Obstet Gynecol170(2):468-73 1994 |
| PubMed ID: 8116699 |
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| Mukherjee AB, Wallace KC, Changes in frequency and localization of human X- and Y-chromatin bodies at interphase during in vitro cellular aging. Mech Ageing Dev53(1):61-71 1990 |
| PubMed ID: 2325441 |
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| Yen PH, Mohandas T, Shapiro LJ, Stability of DNA methylation of the human hypoxanthine phosphoribosyltransferase gene. Somat Cell Mol Genet12:153-61 1986 |
| PubMed ID: 3008350 |
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| Lusis AJ, Heinzmann C, Sparkes RS, Scott J, Knott TJ, Geller R, Sparkes MC, Mohandas T, Regional mapping of human chromosome 19: organization of genes for plasma lipid transport (APOC1, -C2, and -E and LDLR) and the genes C3, PEPD, and GPI. Proc Natl Acad Sci U S A83:3929-33 1986 |
| PubMed ID: 3459164 |
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| Green L, Whittle W, Dell'Orco R, Ostrer H, Stein G, Stein J, Human histone gene organization. Identification of a histone gene polymorphism prevalent in a black population. Exp Cell Res164:507-15 1986 |
| PubMed ID: 3011475 |
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| Nichols WW, Cristofalo VJ, Toji LH, Greene AE, Aronson MM, Dwight S, Charpentier R, Hoffman E, Characterization of a new human diploid cell line--IMR-91. In Vitro19:797-804 1983 |
| PubMed ID: 6629383 |
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| Lieberman MA, Raben D, Glaser L, Cell surface-associated growth inhibitory proteins. Exp Cell Res133:413-9 1981 |
| PubMed ID: 7238608 |
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| Bynum JW, Characterization of adenosine-induced cytostasis in melanoma cells. Cancer Res40:2147-52 1980 |
| PubMed ID: 7388782 |
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| Fischer HD, Gonzalez-Noriega A, Sly WS, Beta-glucuronidase binding to human fibroblast membrane receptors. J Biol Chem255:5069-74 1980 |
| PubMed ID: 6768729 |
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| Brown, Two-dimensional analysis of radiolabeled proteins in cultured Huntington's Disease fibroblasts. Adv Neurol23:361 (1979):5069-74 1979 |
| PubMed ID: 6768729 |
Culture Protocols
| PDL at Freeze |
19 |
| Passage Frozen |
7 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
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