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Overview
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Collection
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NIA Aging Cell Culture Repository
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| Subcollection |
IMR90/IMR91 Cell Cultures
Specially Characterized Fibroblasts |
| Sample Description |
IMR-91 - NORMAL HUMAN FETAL FIBROBLAST |
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Biopsy Source
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Skin
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Species
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Homo sapiens
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Common Name
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Human
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Age At Sampling
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12 FW
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Sex
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Male
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Race
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Caucasian
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Relation to Proband
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proband
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Clinically Affected
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No
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Confirmation
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Clinical summary/Case history
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Remarks
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| Catalog ID |
I91S-07 |
| Product |
Cell Culture |
| Pricing |
Commercial Pricing: $180.00
Academic and not-for-profit pricing: $100.00
NIA Grantees: $40.00 |
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How to Order
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Online Ordering
Assurance Form (Must have current form on file)
Statement of Research Intent Form (Information will be entered electronically when order is placed. DO NOT fax form to Coriell Customer Service)
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Characterizations
| Sample Description |
IMR-91 - NORMAL HUMAN FETAL FIBROBLAST |
| PDL at Freeze |
36 |
| Passage Frozen |
20 |
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Phenotypic Data
| Remark |
The IMR-91 skin-derived line was initiated on 2/24/76 from explants of minced skin tissue from an approximately 12 week fetus. The cell morphology is fibroblast-like. The karyotype is 46,XY; normal diploid male. The HLA type is A2, A11, B18 and B5. G6PD is isoenzyme type B and 6PGD is isoenzyme type A. |
Publications
| Nichols WW, Cristofalo VJ, Toji LH, Greene AE, Aronson MM, Dwight S, Charpentier R, Hoffman E, Characterization of a new human diploid cell line--IMR-91. In Vitro19:797-804 1983 |
| PubMed ID: 6629383 |
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| Brown WT, Ford JP, Gershey EL, Variation of DNA repair capacity in progenia cells unrelated to growth conditions. Biochem Biophys Res Commun97:347-53 1980 |
| PubMed ID: 7458939 |
Culture Protocols
| PDL at Freeze |
36 |
| Passage Frozen |
20 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
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