1. Requirements
2. Procedure
3. Address
4. Fees
5. Shipping Charges
6. Shipment of Cell Cultures
7. Shipment of DNA samples
8. Descriptive Data
9. Request for Citation and Reprint

Cell cultures or DNA are distributed only to qualified professional persons who are associated with recognized research, medical, educational, or industrial organizations engaged in health-related research or health delivery. Before cell cultures or DNA samples can be shipped, the principal investigator must sign an ASSURANCE FORM (Material Transfer Agreement) detailing the terms and conditions of sale. This agreement must be renewed annually. In addition, before receiving lymphoblast or other virus-transformed cell cultures users should read the MINIMUM SAFETY GUIDELINES RECOMMENDED FOR WORKING WITH HUMAN CELL CULTURES. The form and guidelines are available electronically or may be obtained by contacting the Repository.

PROCEDURE

All orders for cell cultures or DNA samples must be submitted using the online catalog. Customers register online and after their registration has been approved, can add items to their shopping cart from the catalog and checkout. See first order for more information. Before requests can be processed, the required information and supporting documentation must be reviewed and approved by the Coriell Order Review Board.

ADDRESS

All Assurance Forms and any accompanying paperwork should be emailed to customerservice@coriell.org

FEES

The pricing will is $85 for one T25 flask of live culture for a cell line and $50 for a 10 µg aliquot of DNA. Purchasers may arrange to take delivery on these cultures on several different dates.

SHIPPING CHARGES

Shipping charges vary depending upon the mode of shipment requested by the purchaser. Domestic shipments are usually made via Federal Express with a guaranteed second day delivery.

SHIPMENT OF CELL CULTURES

All cell cultures are grown and frozen in antibiotic-free media to aid in the detection and prevention of contamination. Cell cultures in the Repository have been tested and found free of mycoplasma, bacteria, and fungi. When an order is received, a frozen ampule is usually recovered from liquid nitrogen on the following Thursday. The culture is inspected for growth and contamination and the medium is changed on Friday. The following Monday, the culture is inspected, the medium is removed, and the T25 flask is filled with fresh medium, packed and shipped. The flask is enclosed within two watertight plastic envelopes within a styrofoam box in a cardboard mailer to prevent leakage, overheating, or freezing during shipment.

Upon receipt, cell cultures should be placed in an incubator at 37°C for a few hours or overnight without removing any medium to permit recovery from damage which may have occurred during shipment. It is suggested that the recipient store aliquots of cells in early passage in liquid nitrogen as insurance against contamination, artifacts associated with aging, and accidents. Additional culture guidelines can be found here.

Frozen ampules are not shipped, because experience has shown much better success in shipping freshly recovered growing cultures. Exceptions must be requested by written application to the CCR Director.

SHIPMENT OF DNA SAMPLES

DNA Samples

DNA samples are recovered from storage at -70°C and shipped on Mondays and Wednesdays at ambient temperature. Recipients should centrifuge the vials prior to opening. Continued storage at -70°C is recommended to maintain the integrity of the DNA.

DNA Quality Control

All DNA samples prepared by the Repository are characterized for the following:

1. OD260 /OD280 ratio, and
2. agarose gel electrophoresis to confirm the integrity and digestibility with Eco R1 and Hind III.

DESCRIPTIVE DATA

Each shipment includes a packing slip and general information about cell cultures or a listing of DNA concentrations. Specific details for each cell line or DNA sample and pertinent reference citations (if available) can be printed from the Coriell Web Catalog. Search the catalog by using the catalog number.

REQUEST FOR CITATION AND REPRINTS

The Repository requests that the Repository number be cited in publications in which cell cultures or DNA from the LMS Repository are used. The Repository would also appreciate receiving a reprint of each publication that references a Repository cell line.